The primary objective of this course is to ensure that each participant, at the end of the training experience, acquires the critical competence and necessary independence to apply the two main multiparametric immunofluorescence techniques proposed, namely Iterative Bleaching EXtends multiplexity (IBEX) and Multiple Iterative Labeling by Antibody Neodeposition (MILAN), dedicated respectively to the analysis of biological samples from murine and human histological sections.

Particular attention will be devoted to the acquisition of a complete mastery of both methodologies, in order to enable students to use these approaches with confidence and precision. Theoretical and, above all, practical insights will be detailed and enriched by laboratory sessions, enabling participants to develop operational and manual skills in the use of instruments and the accurate execution of techniques. 

The acquired skills will enable the individual participant to carry out the methodologies autonomously, developing a high critical sense and problem-solving ability to ensure the reproducibility of these complex methodologies even in contexts and fields of investigation different from those presented in the course. In addition, a key element of the training programme will be to guarantee access to advanced image analysis methodologies, thus enabling course participants to independently manage all stages of the multi-parametric image acquisition and analysis process.

In this practical course, depending on the student’s needs, on the type of tissue and cell population, ad-hoc antibody panels will be provided to stain for B cells, T cells and macrophages.